DNA

Part:BBa_K2557044:Design

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-09)


PhiC31 attP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 17
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 8
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To facilitate cloning, we randomly added some bases on both sides of attP.


Source

After the recombination site of PhiC31 was found in the literature, it was sent to Kingsray for synthesis.

References